Carboxypeptidase Y (CPY) catalyzes the following reaction: Peptidyl-L-amino acid + H2O ——> Peptide + L-amino acid.
It is prepared according to the method of Moore & Stein (J. Biol Chem, 211, 907, 1954). It resembles Carboxypeptidase A in its substrate specificity, but it hydrolyzes C-terminal glycine and L-leuicine more rapidly and L-phenylalanine more slowly.

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